细胞在许多领域中都有广泛的应用。在生物医学研究中，它可以用于研究癌症、感染性疾病、神经退行性疾病等疾病的发生机制和新药的筛选。

在生物技术领域，细胞可以用于生产蛋白质、抗体和疫苗等生物制品。此外，细胞还在组织工程和再生医学中发挥着重要的作用。本期将带大家了解人膀胱癌细胞BIU-87。

1. 细胞常见的研究有哪些？

a) 研究膀胱癌的生物学特性

b) 药物筛选及癌症治疗的研发

c) 有关该癌症细胞耐药机制的研究

d) 建立细胞模型

2. 细胞相关的文献有哪些？

[1] 马燕凌,晏菲,魏武杰等.细胞外囊泡装载多柔比星对人膀胱癌BIU-87细胞增殖及凋亡的影响[J].中国药业,2022,31(14):60-64.

（探讨细胞外囊泡（EVs）装载多柔比星对人膀胱癌BIU-87细胞增殖及凋亡的影响。方法 体外培养BIU-87细胞并进行EVs提取，制备装载多柔比星的载药囊泡。实验分为空白对照组（不进行处理）、EVs组（EVs混悬液，100个/细胞）、多柔比星组（0.3μg多柔比星）、载药囊泡组（载药囊泡混悬液，100个/细胞）。采用MTT法和流式细胞法分别检测细胞增殖率和凋亡率，实时荧光聚合酶链式反应（RT-PCR）法和Western blot法分别检测细胞中糖原合成酶激酶-3β(GSK-3β)、β-连环蛋白（β-catenin）、Notch1的mRNA及蛋白表达水平。结果 EVs组和载药囊泡组细胞内PKH67标记的团块状绿色荧光均匀；与空白对照组比较，EVs组上述指标均无显著差异（P> 0.05）；与空白对照组及EVs组比较，多柔比星组和载药囊泡组细胞增殖率、β-catenin和Notch1 mRNA及蛋白表达水平均显著降低，细胞凋亡率、GSK-3β mRNA及蛋白表达水平均显著升高，且载药囊泡组更优（P <0.05）。结论 EVs装载多柔比星抑制BIU-87细胞增殖和促进其凋亡的作用较单用多柔比星强，机制可能与抑制β-catenin/Notch1信号通路有关。 ）

[2] Cheng XZ, Zhou HL, Tang SX, Jiang T, Chen Q, Gao R, Ding YL. Intercellular transfer of P-glycoprotein mediates the formation of stable multi-drug resistance in human bladder cancer BIU-87 cells. Biol Open. 2019 May 1;8(5):bio041889. doi: 10.1242/bio.041889. PMID: 30967374; PMCID: PMC6550073.

（We investigated the biological characteristics of acquired drug-resistant cells (AqMDRs) formed by intercellular P-glycoprotein (P-gp) transfer and whether AqMDRs can form stable drug-resistant strains. Drug-sensitive BIU-87 cells were co-cultured with doxorubicin (DOX)-resistant derivative BIU-87/DOX cells in transwell chambers for up to 96 h. The presence of P-gp in recipient cell membranes (AqMDRs) was detected by confocal microscopy, CCK-8, western blot, and RT-PCR were used to detect resistance index (RI), P-gp expression and MDR1 mRNA expression in AqMDRs after 0, 4, 8, 16, and 20 passages and frozen/resuscitated twentieth generation AqMDRs. There was an increase in P-gp transfer with longer co-culture times of drug-resistant and sensitive strains. Without DOX, although the AqMDR numbers increased with each passage, the RI and P-gp expression decreased gradually, and the expression level of MDR1 mRNA did not change significantly. With DOX, the RI and P-gp expression increased slightly, and the MDR1 mRNA expression level gradually increased to the BIU-87/DOX level. AqMDRs can grow stably at drug concentrations slightly higher than the IC50 of sensitive strains, which sensitive strains cannot survive. P-gp transfer between cells gradually increases with longer co-culturing of drug-resistant and sensitive strains. The drug resistance of AqMDRs decreases without drug intervention, but with drug intervention, cells can maintain resistance and gradually develop into stable drug-resistant cells. This article has an associated First Person interview with the first author of the paper.）