1. 细胞的基础信息

2. 细胞常见的研究有哪些？

a) 研究肺腺癌的生物学特性

b) 药物筛选及癌症治疗的研发

c) 有关该癌症细胞耐药机制的研究

d) 建立细胞模型

3. 细胞相关的文献有哪些？

[1] 郝艳梅,殷红梅,朱超莽,等.苦参碱通过抑制PI3K/AKT/mTOR通路促进非小细胞肺癌A549细胞的自噬和凋亡[J].南方医科大学学报,2019,39(07):760-765.

（探讨苦参碱对非小细胞肺A549细胞增殖的抑制作用及潜在的分子机制。方法：苦参碱(终浓度为0、0.4、

0.8、1.2、1.6、2.0 g/L)处理非小细胞肺癌A549细胞24、48、72 h,采用CCK-8检测A549细胞的存活情况,荧光显微镜下观察A549细胞的形态学变化,流式细胞术(FCM)分析细胞凋亡情况,Western blot分析苦参碱和PI3K特异性抑制剂LY294002(10 nmol/L)对A549细胞AKT信号通路和自噬相关蛋白的影响。结果苦参碱对A549细胞增殖的抑制作用呈时间-剂量依赖性(P<0.05),当苦参碱浓度达到1.6 g/L时,细胞萎缩加剧,细胞碎片和悬浮细胞明显增多,吖啶橙染色,可以观察到自噬液泡。FCM分析显示随着苦参碱浓度增加,细胞凋亡率也升高,浓度为0.8～1.6 g/L的苦参碱诱导细胞凋亡呈时间和剂量依赖性增加。同时,1.6 g/L苦参碱和LY294002(10 nmol/L)组p-AKT、p-mTOR蛋白表达水平较对照组明显减低(P<0.05),自噬相关轻链蛋白3B(LC 3B)表达水平高于对照组(P<0.05)。结论：苦参碱通过抑制PI3K/AKT/mTOR信号通路的活性,抑制A549细胞增殖,诱导细胞自噬和凋亡,苦参碱可能是一种潜在的肺癌治疗药物。）

[2] Chantragan Srisomsap et al. Pharmacological Properties of White Mulberry (Morus alba L.) Leaves: Suppressing Migratory and Invasive Activities Against A549 Lung Cancer Cells.[J]. Plant foods for human nutrition (Dordrecht, Netherlands), 2024,

（Morus alba known as a white mulberry is a medicinal plant that has been used in food ingredients and traditional medicine. M. alba leaves contain various bioactive phenolic compounds, in particular chlorogenic acid (CGA), which is a major bioactive ingredient. Their anticancer potency of M. alba leaf extracts derived from Soxhlet extraction was evaluated based on cytotoxicity and antimigratory and antiinvasive properties. The dichloromethane extract exhibited the highest nitric oxide radical scavenging activity with a half-maximal inhibitory concentration (IC50) value of 780 μg/mL, promising cytotoxicity against HuCCA-1, MCF-7, and A-549 cells with IC50 values of 59.18, 62.20, and 103.25 μg/mL, respectively. CGA selectively inhibited the growth of MCF-7 cells with an IC50 value of 26.75 μg/mL and showed potent radical scavenging activity against DPPH radicals (IC50 = 18.85 μg/mL). An ethanolic extract derived from the gradient Soxhlet extraction suppressed A549 lung cancer cell migration and invasion more effectively than CGA with no migratory inhibition effect on noncancerous HaCaT cells. Furthermore, the ethanolic extract and CGA accelerated HaCaT wound closure at 20 µg/mL, which was the same as allantoin. Bioactive ingredients including triterpenes, steroids, phenolics, and flavonoids were mainly detected in all extracts. The highest content of CGA (52.23 g/100 g dry weight) was found in the ethanolic extract derived from the gradient Soxhlet extraction. These findings show the potency of the dichloromethane extract as a cytotoxic agent against various cancer types and the ethanolic extract as an antimetastatic agent by their antimigratory and antiinvasive activities.）